|
种属来源 |
人 |
|
别称 |
H292; H-292; NCI-HUT-292; Hut292; NCIH292 |
|
组织来源 |
肺 |
|
疾病特征 |
粘液表皮样肺癌 |
|
细胞形态 |
上皮细胞样 |
|
生长特性 |
贴壁生长 |
|
倍增时间 |
~48h |
|
致瘤性 |
Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features. |
|
基因表达 |
keratin; vimentin,The cells retain their mucoepidermoid characteristics in culture as determined by their ultrastructure and expression of multiple markers of squamous differentiation. TANK cells show reactivity with human alloantisera specific for the HLA A1, A3, A10, A19, B12, B17, B22 and B40 cross-reactive groups. |
|
STR |
Amelogenin:X;CSF1PO:10;D13S317:11,12;D16S539:9,13;D18S51:16;D19S433:12,13.2;D21S11:28;D2S1338:21,24;D3S1358:16;D5S818:13;D7S820:10;D8S1179:11,14;FGA:22,26;TH01:8;TPOX:8,11;vWA:16; |
|
培养体系 |
RPMI1640培养基;10%胎牛血清;1%双抗 |
|
传代方法 |
1:2-1:3传代,消化2-3分钟 |
|
培养条件 |
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液:90%FBS,DMSO 10%, 更推荐无血清细胞冻存液:货号CC1-100 |
|
保藏机构 |
ATCC; CRL-1848 |
|
产品用途 |
仅限科研使用。 |