|
种属来源 |
人 |
|
别称 |
HT 29; HT29 |
|
组织来源 |
结肠 |
|
疾病特征 |
结肠直肠腺癌 |
|
传代比例/细胞消化 |
1 :2传代 ,消化3-5分钟 |
|
细胞形态 |
上皮细胞样 |
|
生长特性 |
贴壁生长 |
|
倍增时间 |
~40-60h |
|
受体表达 |
human adrenergic alpha2A, urokinase receptor (u-PAR), vitamin D (moderate expression), urokinase receptor (u-PAR); vitamin D (moderate expression), human adrenergic alpha2A |
|
基因表达 |
secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin, myc+; ras+; myb +; fos+; sis+; p53+; abl -; ros -; src -, Blood Type A; Rh +; HLA A1, A3, B12, B17, Cw5, HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). |
|
致瘤性 |
Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters. |
|
STR |
Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 10 TH01: 6,9 TPOX: 8,9 vWA: 17,19 |
|
培养体系 |
DMEM培养基;10%胎牛血清;1%双抗 |
|
培养条件 |
气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液 :90%FBS ,DMSO 10%, 或使用非程序冻存液 :官网货号CC1-100 |
|
保藏机构 |
ATCC; HTB-38 |
|
产品用途 |
仅限于科学研究 ,不可作为动物或人类疾病特征的治疗产品使用。 |