|
种属来源 |
人 |
|
别称 |
Hpac |
|
组织来源 |
胰腺 |
|
疾病特征 |
胰腺癌 |
|
细胞形态 |
上皮细胞样 |
|
生长特性 |
贴壁生长 |
|
倍增时间 |
~48h |
|
基因表达 |
HPAC cells are positive for keratin and negative for vimentin and chromogranin A. |
|
抗原表达 |
epidermal growth factor (EGF), expressed; glucocorticoid, expressed; epidermal growth factor (EGF); glucocorticoid |
|
致瘤性 |
Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin. Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose. |
|
STR |
Amelogenin:X;CSF1PO:13;D13S317:11;D16S539:9,10;D18S51:16;D19S433:14;D21S11:30;D2S1338:19,23;D3S1358:15,17;D5S818:12;D7S820:10,12;D8S1179:12,14;FGA:24;TH01:9.3;TPOX:10,11;vWA:15,17; |
|
培养体系 |
DMEM培养基;10%胎牛血清;1%双抗 |
|
传代方法 |
1:2-1:3传代,消化3-5分钟, |
|
培养条件 |
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液:90%FBS,DMSO 10%, 更推荐无血清细胞冻存液:货号CC1-100 |
|
保藏机构 |
ATCC; CRL-2119 |
|
产品用途 |
仅限科研使用。 |