|
种属来源 |
小鼠 |
|
别称 |
bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3 |
|
组织来源 |
大脑皮层 |
|
疾病特征 |
内皮瘤 |
|
传代比例/细胞消化 |
1:2传代 ,消化2-3分钟 |
|
细胞形态 |
上皮细胞样 |
|
生长特性 |
贴壁生长 |
|
倍增时间 |
~24-32h |
|
基因表达 |
von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd |
|
抗原表达 |
ICAM-1 +; VCAM-1 +; MAdCAM-1 + |
|
培养体系 |
DMEM( 含NaHCO3 1.5g/L)培养基;10%胎牛血清;1%双抗 |
|
培养条件 |
气相:空气 ,95% ;二氧化碳 ,5%。 温度:37摄氏度 ,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液:90%FBS ,DMSO 10%, 更推荐无血清细胞冻存液:货号CC1-100 |
|
保藏机构 |
ATCC; CRL-2299 |
|
产品用途 |
仅限于科学研究 ,不可作为动物或人类疾病特征的治疗产品用途。 |